Journal: Diabetes
Article Title: Identifying Insulin Autoantibodies With Differential Risk in Type 1 Diabetes With a Novel Bridging ELISA
doi: 10.2337/db25-0696
Figure Lengend Snippet: Schematic of ELISA-IAA assay design. The bridging ELISA-IAA assay is designed for IAA to bind two differently labeled proinsulin molecules in the fluid phase. The antigen (proinsulin)-antibody (IAA) complex generates a measurable signal through enzyme-substrate reactions, which are detected using a standard ELISA plate reader. mAb, monoclonal antibody; SA, streptavidin; TMB, 3,3′,5,5′-tetramethylbenzidine (peroxidase substrate).
Article Snippet: HRP-conjugated streptavidin (Thermo Fisher Scientific) is added and incubated at room temperature for 1 h. After additional washes, the peroxidase substrate 3,3′,5,5′-tetramethylbenzidine (Thermo Fisher Scientific) is added to develop color.
Techniques: Enzyme-linked Immunosorbent Assay, Labeling